Recently, "Nature-Methodology" introduced a method that can produce error-free long DNA sequences containing thousands of base pairs. The method uses single-molecule real-time (SMRT) sequencing technology and simplifies the assembly process of the bacterial genome without reference to the sequencing group, in order to better understand the role of the bacterial genome in ecology and pathology and to track its evolution.
Bacterial genomes are usually composed of short DNA sequence fragments generated by second-generation sequencing equipment, but there are some gaps in the genome generated in this way that are difficult to fill. Some recently developed hybrid methods combine different sequencing technologies and fill the gaps left by the assembly of short sequences, such as those generated by conventional second-generation sequencing equipment and those generated by SMRT sequencing technology, although the accuracy is poor, But the gap caused by the information of longer sequence fragments. This method requires complex sequencing libraries and sequencing equipment.
Many sequencing applications have always had a high demand for long sequences, but the error rate limits their utility. The method designed by Jonas Korlach et al. Uses short sequence fragments in the same sequence library to correct errors in long sequence fragments, so that the assembly of bacterial genomes and artificial bacterial chromosomes has the advantages of high quality and no gaps in the automatic assembly line. At the same time, this method also effectively solves some problems in repeated sequencing.
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