Experimental principle of ELISA kit for neurotrophic factor (GDNF) derived from human glial cell line
English name: Human glial cell line-derived neurotrophic factor, GDNF ELI
1. The level of neurotrophic factor (GDNF) ELISA Kit derived from human glial cell line in the specimen was determined by double antibody sandwich method.
2. First, coat the microplate with purified human glial cell line-derived neurotrophic factor (GDNF) ELISA Kit antibody to prepare a solid-phase antibody.
3. Then add glial cell line-derived neurotrophic factor (GDNF) ELISA Kit to the microwells coated with mAb in turn, and then combine with HRP-labeled glial cell line-derived neurotrophic factor (GDNF) ELISA Kit antibody, An antibody-antigen-enzyme-labeled antibody complex is formed, and after thorough washing, a substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid.
4. The color depth is positively correlated with the neurotrophic factor (GDNF) ELISA Kit derived from the glial cell line in the sample.
5. Finally, the absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of the neurotrophic factor (GDNF) ELISA Kit derived from human glial cell line in the sample was calculated from the standard curve.
Objective: To determine the content of glial cell line-derived neurotrophic factor (GDNF) ELISA Kit in human serum, plasma and related liquid samples.
6. Serum: Blood coagulates naturally at room temperature for 10-20 minutes, centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully and centrifuge again if a precipitate appears during storage. Avoid any cell stimulation during the operation. Use test tubes free of pyrogens and endotoxins.
7. Plasma: EDTA or sodium citrate should be selected as the anticoagulant according to the requirements of the specimen. After mixing for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm) to remove particles. Collect the supernatant carefully, and centrifuge again if there is any precipitate.
safety:
1. Avoid direct contact with stop solution and substrate. If you accidentally come into contact with these liquids, please rinse them with water as soon as possible.
2. Do not eat, drink, smoke or use cosmetics during the experiment.
3. Do not touch any ingredients in the kit with your mouth.
4 Keep Elisa kit away from children's own items:
1. 37 ℃ incubator
2. Standard specification microplate reader
3. Precision pipette and disposable tip
4. Distilled water
5. Disposable test tube
6. Absorbent paper
Storage conditions and validity period:
1. Kit storage :; 2-8 ℃.
2. Validity: 6 months Product range:
Human, mouse, rat, pig, rabbit, other animal cytokines; apoptosis, active peptides, autoantibodies, thrombosis and hemostasis,
Bone metabolism, liver fibrosis, tumor, hormone endocrine, autoantibody scientific research ELISA test kit
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