Latex agglutination test and gelatin agglutination test

Latex agglutination test and gelatin agglutination test

Latex agglutination test (LATExagglutinationtest, LAT) is also an indirect agglutination test, the carrier particles used are polystyrene latex, a diameter of about o. 8flm-sized round particles, with a negative charge, can physically adsorb protein molecules, but the binding is poor. It can also be prepared as particles with chemically active groups, such as carboxylated polystyrene latex with carboxyl groups. Antigens or antibodies are cross-linked on the latex surface with covalent bonds. Chemical cross-linking—Generally, the carboxyl group on the latex and the amino group on the cross-linked product are condensed together through the condensation agent carbodiimide. This latex reagent sensitized with cross-linking has stable performance and long shelf life.

Latex agglutination test is divided into test tube method and slide method. In the test tube method, the test specimen is first diluted in a test tube with a buffer solution, and then the sensitized latex reagent is added. After the reaction, the latex agglutination is observed. The slide method is easy to operate. After one drop of the specimen and one drop of sensitized latex reagent are mixed on the slide, the result can be observed by continuously shaking for 2 minutes for 3 minutes. Agglomeration of large particles is a positive reaction; maintaining a uniform emulsion is a negative reaction. Latex is a synthetic carrier, so its performance is more stable and uniform than that of biologically derived red blood cells. However, the binding capacity and agglutination performance of latex and protein are not as good as those of red blood cells. Therefore, as an indirect agglutination test, the sensitivity of the latex test is not as good as the hemagglutination test.

The white latex was observed with little contrast during the latex agglutination test. In order to improve the sensitivity of the latex, a variety of nitrogen-containing color latexes were prepared. The color Schiff base was reduced and reacted with acrylic acid to prepare color monomers. The body is copolymerized with styrene and purified by dialysis to obtain a pink latex. This latex is bound to an antibody to detect the corresponding antigen, and the sensitivity is 10ug / m1.

The gelatin agglutination test (GAT) is also an indirect agglutination test, which

The toxic antigen or recombinant antigen is adsorbed on the pink gelatin particles. When the sensitized particles interact with the sample serum, if the serum contains antiviral antibodies, it can form a pink agglutination visible to the naked eye. This method has the advantages of high sensitivity, simplicity, and speed, and is widely used in the detection of HIV-1 antibodies and anti-sperm antibodies.

Applicable to any brand of Offset Printing press,suitable for UV printing.It`s completely free of formaldehyde, the plate has longer service life.The best ink-water balance,Reduce ink accumulation on blanket surface,reduce washing time.The stability of the ink-water balance,reduce ink emulsification ,ink surface moisture reduction,effectively control roll surface to produce calcium.

UV Fountain Solution

Uv Offset Printin,Uv Fountain Solution,Journal Fount,Fountain Solution For Offset Printing

Shanghai Chenjie Printing Material Co., LTD , https://www.shprintbar.com