Rat granulocyte-macrophage colony stimulating factor (GM-CSF)
Instruction manual for ELISA kit
Read this manual carefully before use. This ELISA kit is based on the principle of double antibody sandwich technology to detect rat granulocyte-macrophage colony stimulating factor (GM-CSF), which can only be used for research purposes and not for medical diagnosis.
Use: Used for the determination of granulocyte-macrophage colony stimulating factor (GM-CSF) in rat serum, plasma and related liquid samples.
working principle
This kit uses biotin double antibody sandwich enzyme-linked immunosorbent assay (ELISA) to determine the level of rat granulocyte-macrophage colony stimulating factor (GM-CSF) in the sample. Add rat granulocyte-macrophage colony stimulating factor (GM-CSF) to the enzyme-labeled wells pre-coated with the monoclonal antibody of rat granulocyte-macrophage colony stimulating factor (GM-CSF) and incubate; After incubation, biotin-labeled anti-GM-CSF antibody was added. It is combined with streptavidin-HRP to form an immune complex. After incubation and washing, the unbound enzyme is removed, and then substrates A and B are added to produce a blue color, which is converted into the final under the action of acid Yellow. The depth of the color is positively related to the concentration of rat granulocyte-macrophage colony stimulating factor (GM-CSF) in the sample
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