Rat nerve growth factor content Specification Purpose: This kit is used to determine the content of nerve growth factor (NGF) in rat serum, plasma and related liquid samples. Experimental principle: This kit uses the double antibody sandwich method to determine the level of rat nerve growth factor (NGF) in the specimen. The microplate was coated with purified rat nerve growth factor (NGF) antibody to prepare a solid phase antibody, and nerve growth factor (NGF) was sequentially added to the microcapsule of the coated monoclonal antibody, followed by HRP-labeled nerve growth factor ( The NGF) antibody binds to form an antibody-antigen-enzyme-labeled antibody complex, which is thoroughly washed and then added to the substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The depth of the color is positively correlated with the nerve growth factor (NGF) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of rat nerve growth factor (NGF) in the sample was calculated from a standard curve.
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