Use of lactose bile salt fermentation medium and its use method


Used for multi-tube fermentation method to determine coliform, fecal coliform and Escherichia coli in food, purified water and disposable sanitary products (GB / T 8538-2008 and GB15979-2002). Principle: Peptone provides carbon and nitrogen sources to meet the needs of bacterial growth; pig bile salts can inhibit the growth of Gram-positive bacteria; lactose is a fermentable sugar of coliform bacteria; bromocresol purple is a pH indicator, acidic Yellow, alkaline is purple. Recipe (per liter):

Peptone 20g Pig bile salt 5g Lactose 10g Bromocresol purple 0.01g

Final pH 7.4 ± 0.2 How to use: 1. Weigh 35g (single material) or 70g (double material) of this product, add 1L of distilled water or deionized water, stir and heat to boil until completely dissolved, and dispense it into a small inverted tube In the test tube, the culture medium was 10mL per tube and autoclaved at 115 ° C for 15min. 2. Sample processing and dilution. slightly. 3. (1) Food: Take 1mL of diluent and add it to a test tube containing 10mL of lactose bile salt medium. According to the requirements of food hygiene standards or the estimation of the contamination of the test sample, select three suitable dilutions for each dilution Inoculate three tubes. Inoculate the sample to be tested in the lactose bile salt fermentation tube. If the inoculation volume is more than 1 mL, use a double-feed lactose bile salt fermentation tube. If the inoculation amount is less than 1 mL, use a single lactose bile salt fermentation tube. (2) Disposable hygiene products: 5mL of sampling solution is inoculated into a 50mL single material lactose bile salt fermentation tube. 4. Put the test tube into the incubator at 36 ± 1 ℃ for 24 hours. 5. Observe the results. If all lactose bile salt fermentation tubes do not produce gas, it can be reported as negative for coliform bacteria. If there is gas production, separation and confirmation tests are required. Quality control: The prepared medium is purple. After the quality control strains are inoculated, they are cultured at 36 ± 1 ℃ for 24h. The results are as follows:

Bacteria name growth status Acid-producing gas-generating Escherichia coli ATCC25922 good + + Staphylococcus aureus ATCC6538 is inhibited--

Storage: Store in a cool, dry place away from light. Tighten the cap immediately after use. The storage period is three years.

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