This kit is for research use only. Detection range: 96T1μmol / L-32μmol / L Purpose: This kit is used to determine the content of β-hydroxybutyric acid (β-OHB) in human serum, plasma and related liquid samples. Experimental principle The kit uses the double antibody sandwich method to determine the level of human β-hydroxybutyric acid (β-OHB) in the specimen. Microporous plates were coated with purified human β-hydroxybutyric acid (β-OHB) antibody to make solid-phase antibodies, and β-hydroxybutyric acid (β-OHB) was added to the microwells coated with mAb in turn, followed by HRP label The β-hydroxybutyric acid (β-OHB) antibody binds to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with β-hydroxybutyric acid (β-OHB) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of human β-hydroxybutyric acid (β-OHB) in the sample was calculated by a standard curve. Kit composition 1 30-fold concentrated washing solution 20ml × 1 bottle 7 stop solution 6ml × 1 bottle 2 enzyme label reagent 6ml × 1 bottle 8 standard product (64μmol / L) 0.5ml × 1 bottle 3 enzyme label coated plate 12 well × 8 strips 9 standard diluent 1.5ml × 1 bottle 4 sample diluent 6ml × 1 bottle 10 instruction manual 1 copy 5 developer A solution 6ml × 1 bottle 11 sealing film 2 sheets 6 developer B solution 6ml × 1 / Bottle 12 sealed bag 1 specimen requirement 1. Specimens are extracted as soon as possible after collection, and extraction is performed according to relevant literature, and experiments should be conducted as soon as possible after extraction. If the test cannot be performed immediately, the specimen can be stored at -20 ℃, but repeated freezing and thawing should be avoided. The samples containing NaN3 cannot be detected because NaN3 inhibits horseradish peroxidase (HRP) activity. Procedure 1. Dilution of standard product: This kit provides one original standard product. The user can perform dilution in a small test tube according to the following chart. 32μmol / L No. 5 standard 150μl original standard added 150μl standard dilution 16μmol / L No. 4 standard 150μl No. 5 standard added 150μl standard dilution No. 8 μmol / L No. 3 standard 150μl No. 4 Add 150μl standard dilution 4μmol / L No. 2 standard 150μl No. 3 standard add 150μl standard dilution 2μmol / L No. 1 standard 150μl No. 2 standard add 150μl standard dilution 2. Add sample: Separately set blank wells (the blank control wells do not add samples and enzyme label reagents, the rest of the operation is the same), standard wells, sample wells to be tested. Accurately add 50μl of the standard on the enzyme-coated plate, add 40μl of sample diluent to the sample well, and then add 10μl of the sample to be tested (the final dilution of the sample is 5 times). Add the sample and add the sample to the bottom of the well of the microplate, try not to touch the wall of the well, shake gently to mix. 3. Incubation: Seal the plate with a sealing plate and incubate at 37 ° C for 30 minutes. 4. Mixing solution: Dilute the 30-fold concentrated washing solution with distilled water 30-fold and reserve for use. 5. Washing: Carefully remove the sealing film, discard the liquid, spin dry, fill each well with the washing solution, and let it stand for 30 seconds before discard , Repeat this 5 times, pat dry. 6. Add enzyme: add 50μl of enzyme label reagent to each well, except blank well. 7. Incubation: The operation is the same as 3. 8. Washing: The operation is the same as 5. 9. Color development: add 50μl of developer A to each well, then add 50μl of developer B, mix gently, and develop color at 37 ° C in the dark 15 minutes. 10. Stop: Add 50μl of stop solution to each well to stop the reaction (the blue color turns to yellow at this time). 11. Determination: Measure the absorbance (OD value) of each well in sequence with the blank air conditioner at zero and 450 nm wavelength. The measurement should be carried out within 15 minutes after adding the stop solution.
The makeup brushes in this category are for Phoenix Beauty brand. The makeup brush sets are consistent of some basical brushes for makeup, such as powder brush, blush brush, highlighter brush, foundation brush, eye shadow brush and blending brush, so they are good choices for both makeup artists and makeup beginners. Brushes with goat hair is perfect for application of powder products, so most of the brushes for application of powder products are made of goat hair. Synthetic hair is durable and easy to clean, and perform well for application of liquid products, so the concealer brush and foundation brush are made of synthetic hair.
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