Rat 11 dehydrotherosin B2 ELISA kit Experimental method Description Detection principle The kit uses a double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). To the coated microwells pre-coated with the dehydrothromboxane B2 (11-DH-TXB2) capture antigen of the rat 11, the specimen, the standard, and the HRP-labeled detection antibody were sequentially added, and the cells were washed and thoroughly washed. Using the substrate TMB to develop color, TMB is converted to blue under the catalysis of peroxidase and converted to the final yellow color by the action of an acid. The color depth is positively correlated with rat 11 dehydrothromboxane B2 (11-DH-TXB2) in the sample. The absorbance (OD value) was measured at 450 nm using a microplate reader to calculate the sample concentration.
Beijing Jiehui Bio provides free testing service: using our company's kit, only the cost of the kit is charged, and the testing fee is free. Based on the experimental workload and difficulty level, we will agree on the experimental cycle and complete the entrusted experiment with quality and quantity.
Rat 11 dehydrotherosin B2 ELISA kit experimental method to illustrate sample collection, processing and preservation methods
1. Serum: Use a tube containing no pyrogen and endotoxin. Avoid any cell irritation during the procedure. After collecting the blood, centrifuge and centrifuge for 10 minutes at 3000 rpm to quickly and carefully separate the serum and red blood cells.
2. Plasma: EDTA, citrate or heparin anticoagulation. The supernatant was taken by centrifugation at 3000 rpm for 30 minutes.
3. Cell supernatant: Centrifuge at 3000 rpm for 10 minutes to remove particles and polymer.
4. Tissue homogenization: The tissue is added to the appropriate amount of physiological saline and chopped. The supernatant was taken by centrifugation at 3000 rpm for 10 minutes.
5. Storage: If the sample is not detected in time after collection, please dispense it once, freeze it at -20 °C, avoid repeated freezing and thawing, thaw at room temperature and ensure that the sample is fully thawed evenly.
Solemnly declare:
Please ask the customer to communicate with our sales technicians before taking the materials. The previous materials will directly affect your experimental results. We have a complete set of pre-sales and after-sales service. If you are not purchasing ELISA products in our company, our company will not be responsible for after-sales service.
Rat 11 dehydrotherosin B2 ELISA kit experimental method description steps
1. Remove the required slats from the foil pouch after equilibrating for 20 min at room temperature. The remaining slats are sealed back to 4 °C with a ziplock bag.
2. Set standard hole, sample hole and blank hole, blank hole is added; standard product hole is added with different concentration of standard product 50μL;
3. The sample hole to be tested is first added with 10 μL of the sample to be tested, and then 40 μL of the sample diluent is added;
4. Then add 50 μL of horseradish peroxidase (HRP)-labeled detection antibody to the standard well and sample well (without blank). Seal the well with a sealing membrane, 37 ° C water bath or incubator temperature Breed for 60min.
5. Discard the liquid, pat dry on the absorbent paper, fill each well with the washing solution, let stand for 1 min, remove the washing solution, pat dry on the absorbent paper, and repeat the washing 5 times (can also be washed with a washing machine).
6. Add 50 μL of substrate A and B to all wells and incubate for 15 min at 37 ° C in the dark.
7. Add 50 μL of stop solution to all wells and measure the OD value of each well at a wavelength of 450 nm within 15 min.
Beijing Jiehui Biotechnology Co., Ltd. free test ELISA kit within one week results 010-57269780
Description of experimental method for rat 11 dehydrothorphane B2 ELISA kit
Human Alanine Aminotransferase, ALT/GPT ELISA Kit Human alanine aminotransferase/alanine aminotransferase (ALT/GPT) ELISA Kit
Human malondialchehyche, MDA ELISA Kit Human Malondialdehyde (MDA) ELISA Kit
Human Complement 3, C3 ELISA kit Human Complement 3 (C3) ELISA Kit
Human asymmetrical dimethylarginine, ADMA ELISA Kit Human Asymmetric Dimethylarginine (ADMA) ELISA Kit
Human Brucella IgG, igG ELISA kit Human Brucella igG ELISA Kit
Human Laminin, LN ELISA KIT Human Laminin/Laminin (LN) ELISA KIT
Human Intestinal trefoil factor, ITF ELISA Kit Human Intestinal Trefoil Factor (ITF) ELISA Kit
Human intestinal fatty acid binding protein, iFABP ELISA Kit Human intestinal fatty acid binding protein (iFABP) ELISA Kit
Human Super Oxidase Dimutase, SOD ELISA Kit Human Superoxide Dismutase (SOD) ELISA Kit
Human maturation promoting factor, MPF ELISA Kit Human maturation promoting factor (MPF) ELISA Kit
Human Perforin/Pore-forming protein, PF/PFP ELISA Kit Human Perforin/Poreolin (PF/PFP) ELISA Kit
Human pituitary adenylate cyclase activating polypeptide, PACAP ELISA Kit Human pituitary adenylate cyclase activating peptide (PACAP) ELISA Kit
Human Estriol, E3 ELISA Kit Human Estriol (E3) ELISA Kit
Human corticotropin-releasing factor, CRF Human Corticotropin Releasing Factor (CRF) ELISA Kit
Human delta sleep-inducing peptide,DSIP ELISA Kit Human Sleep-Promoting Peptide (DSIP) ELISA Kit
Human gastrin receptor, GsaR ELISA Kit Human Gastrin Receptor (GsaR) ELISA Kit
Human Secretin ELISA Kit Human Secretin/Secretin ELISA Kit
Human colon cancer-specific antigen-2, CCSA-2 ELISA kit Human colorectal cancer specific antigen 2 (CCSA-2) ELISA Kit
Human colon cancer-specific antigen-3, CCSA-3 ELISA kit Human colorectal cancer specific antigen 3 (CCSA-3) ELISA Kit
Human colon cancer-specific antigen-4, CCSA-4 ELISA kit Human colorectal cancer specific antigen 4 (CCSA-4) ELISA Kit
Human type A monoamine oxidase, MAO-A, ELISA KIT Human monoamine oxidase A (MAOA) ELISA Kit
Human Herpes simplex virus, HSV ELISA kit Human Herpes Simplex Virus (HSV) ELISA Kit
Human monocyte chemotactic protein 1/monocyte chemotactic and activating factor, MCP-1/MCAF ELISA kit Human monocyte chemoattractant protein 1 (MCP-1/CCL2/MCAF) ELISA Kit
Human monocyte chemotactic protein 2,MCP-2 ELISA kit Human monocyte chemoattractant protein 2 (MCP-2/CCL8) ELISA Kit
Human monocyte chemotactic protein 3,MCP-3 ELISA kit Human monocyte chemoattractant protein 3 (MCP-3/CCL7) ELISA Kit
Human monocyte chemotactic protein 4,MCP-4 ELISA kit Human monocyte chemoattractant protein 4 (MCP-4/CCL13) ELISA Kit
Human cholest erolester transfer protein/lipid transfer protein, CETP/LTP ELISA Kit Human cholesterol ester transporter (CETP) ELISA Kit
Human Cholecystokinin, CCK ELISA Kit Human Cholecystokinin (CCK) ELISA Kit
Human cholecystokinin, CCK ELISA Kit Human cholecystokinin/incretin peptide (CCK) ELISA KIT
Human cholecystokinin octapeptide, CCK-8 ELISA Kit Human cholecystokinin/Cholecystokinin octapeptide (CCK-8) ELISA Kit
Glass Perfume Bottles,Blown Glass Perfume Bottle,Antique Glass Perfume Bottles,Vintage Glass Perfume Bottles
Zhejiang Changxing Hanghua Glass Co., Ltd. , https://www.hanghua-glass.com