**Technical Specifications and Elisa Principles for Various Kits**
This document provides an overview of the technical specifications, principles, and step-by-step procedures for a range of ELISA kits produced by Guangrui Bio, a leading domestic supplier of high-quality and excellent ELISA kits. These kits are designed for the detection of various biomarkers in different species, including mice, humans, chickens, fish, and pigs.
The **mouse annexin V (ANX-V) ELISA** is based on the principle of competitive binding between the antibody and the antigen. The procedure involves coating the microtiter plate with anti-annexin V, adding the sample, and then detecting the bound annexin V using a labeled secondary antibody.
For the **human pancreaticin (Pancreastatin) ELISA**, the method follows a standard sandwich ELISA format. The target antigen is captured by a specific primary antibody, followed by detection with a secondary antibody conjugated to an enzyme. This allows for the quantification of pancreaticin levels in serum or plasma samples.
The **human endotoxin (ET) ELISA** is used to detect endotoxins, primarily lipopolysaccharides (LPS), in biological samples. The assay uses a monoclonal antibody that specifically recognizes LPS, making it highly sensitive and suitable for clinical and research applications.
In the case of **chicken soluble vascular endothelial cell protein C receptor (sEPCR)**, the ELISA kit follows a similar sandwich principle. The sEPCR antigen is captured by a specific antibody, and the detection is performed using a labeled secondary antibody, enabling accurate quantification of this important receptor in avian samples.
The **human soluble leptin receptor (sLR)** ELISA is another key tool for studying leptin signaling pathways. The technique involves immobilizing the receptor on a solid surface, incubating with the sample, and then detecting the bound receptor using a specific antibody.
For **fish triiodothyronine (T3) ELISA**, the method utilizes a competitive immunoassay. T3 in the sample competes with a labeled T3 for binding to a specific antibody, allowing for precise measurement of thyroid hormone levels in aquatic species.
The **mouse anti-nuclear membrane glycoprotein 210 (gp210) ELISA** is used to detect autoantibodies against nuclear envelope proteins. This test is particularly useful in diagnosing autoimmune disorders such as systemic lupus erythematosus (SLE).
The **human bone growth peptide (OGP) ELISA** is designed to measure the levels of this bioactive peptide involved in bone remodeling. The protocol includes coating the plate with a specific antibody, adding the sample, and detecting the bound OGP using a chromogenic substrate.
The **Elek’s plant vitamin B12 (VB12) ELISA** is used to quantify vitamin B12 levels in plant-based samples. This method is essential for nutritional studies and food analysis.
The **human macrophage inflammatory protein 1β (MIP-1β/CCL4) ELISA** is employed to measure inflammatory cytokine levels in human samples. The procedure follows a standard sandwich ELISA approach, ensuring high specificity and sensitivity.
For **mouse aquaporin 2 (AQP-2) ELISA**, the method detects the water channel protein AQP-2, which is crucial for kidney function. The assay uses a specific antibody to capture and detect AQP-2 in tissue or fluid samples.
The **mouse bone morphogenetic protein receptor II (BMPR-II) ELISA** is used to assess the expression of this receptor, which plays a key role in bone development and differentiation.
The **mouse factor VIII-related antigen (FVIII-Ag) ELISA** is utilized to measure coagulation factor VIII levels, an important marker in hemostasis and thrombosis studies.
Finally, the **porcine antithrombin receptor (ATR) ELISA** is designed for detecting the interaction between antithrombin and its receptor in porcine samples, aiding in the study of coagulation mechanisms.
Each of these ELISA kits comes with a reagent box containing 50% egg yolk lotion (5ml*10), ensuring stability and optimal performance during the testing process.
These kits are ideal for researchers and clinicians looking for reliable, accurate, and easy-to-use tools for biomarker detection across multiple species.
Wallet,Purses For Women,Card Bag,Coins Bag
NINGBO SUREWAY E-FASHION IMPORT AND EXPORT CO,.LTD , https://www.surewaybags.com